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X射线辐射对仔鼠肝组织结构和相关蛋白的影响 被引量:2

Effects of X-ray on Histological Structure and Interrelated Polypeptides in Liver of Filial Mice
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摘要 为探讨X射线辐射对仔鼠肝重量、肝组织结构及肝中Bax蛋白表达的影响,对160只仔鼠(出生6—7d)。用不同剂量(0、1、3、5、7Gy)的X射线进行全身辐射,分别于辐射后1、5、10、20d,检测各期仔鼠肝重和体重的变化,用显微技术观察仔鼠肝脏组织结构的变化,用免疫组织化学方法检测肝脏中Bax蛋白的表达。结果表明,X射线辐射影响仔鼠肝脏的生长发育。辐射后5—20d,辐射组仔鼠肝重均低于对照组。差异显著(P〈0.05)或极显著(P〈0.01):除1Gy辐射组外,其他辐射组在5-20d时仔鼠体重均低于对照组.差异极显著(P〈0.01);随辐射剂量增大,辐射组仔鼠肝中Bax蛋白阳性表达逐渐增强;辐射后仔鼠肝小叶结构不清,肝板变细,肝血窦变大,肝细胞变形,排列不规则。细胞核萎缩,细胞出现空泡化。 To explore the effects of X-ray on weight, histological structure and expression of Bax protein in liver of developing filial mice, 160 mice of 6-7 days after birth were radiated with different dosages (0, 1, 3, 5, 7Gy) of X-ray. Changes of liver and body weight were examined, histological structure was observed using bio-microscopy,and the expression of Bax protein was measured by immunohistochemical staining in filial mice liver at 1, 5, 10 and 20d after irradiation. Results show that X-ray influences the growth and development of the filial mice liver. The liver weight of filial mice of the experimental groups is lower than that of the control group 5-20d after irradiation (P〈0.05 or P〈0.01), except experimental groups with 1Gy; the body weight of filial mice of the experimental groups is lower than that of the control group 5-20d after irradiation (P〈0.01); the expression intensity of Bax protein in the liver of filial mice is significantly increased with the increase of the dose; the structure of hepatic lobule is unclear, hepatic plate is thinner, hepatic sinusoid is stronger, accompanied with hepatocyte disfiguration, irregular arrangement, nucleus atrophy, and vacuolization, after irradiation.
出处 《科技导报》 CAS CSCD 北大核心 2010年第13期87-91,共5页 Science & Technology Review
基金 国家自然科学基金项目(30370163) 甘肃省创新人才基金项目(ZS006-016)
关键词 X射线 仔鼠 组织结构 BAX蛋白 X-ray filial mice liver histological structure Bax protein
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  • 1田志杰,沈南,吕士杰,王程,姜艳霞,李妍,罗军.高强度微波辐射对Wistar大鼠血清SOD、HSP70和肝脏MDA、Mit肿胀的影响[J].四川动物,2009,28(4):528-531. 被引量:15
  • 2Guryev D V.Histologic assessment of regenerating rat liver under lowdose rote radiation exposure[J].International Congress Series,2005,1276:181-182.
  • 3Zhao Jiandong,Guo Liangjiang,Wei Ganghu,et al.Hepatecyte regeneration after partial liver irradiation in rots[J].Experimental and Toxicologic Pathology,2009,61:511-518.
  • 4Christiansen H,Saile B,Neubauer-Saile K,et al.Irradiation leads to susceptibility of hepatocytes to TNF-a mediated apoptosis[J].Radiotherapy and Oncology,2004,72:291-296.
  • 5邓海平,俞诗源,陈玉琴,王昱,郭婷婷,冯红丽,贾艳芳.X射线辐射对仔鼠体重、皮毛生长及肝、肾脏组织SOD、CAT活力及MDA含量的影响[J].动物学报,2008,54(6):1029-1037. 被引量:23
  • 6Chung H C,Kim S-H,Lee M G,et al.Mitochondrial dysfunction by γ-irradiation accompanies the induction of cytochrome P4502E1(CYP2E1)in rat liver[J].Toxicology,2001,161:79-91.
  • 7Okuno S,Saito A,Hayashi T,et al.The c-Jun N-terminal protein kinase signaling pathway mediates Bax activation and subsequent neuronal apoptosis through interaction with Bim after transient focal cerebral ischemia[J].J Neurosci,2004,24(36):7879-7887.
  • 8Shibata M,Murray M,Tessler A,et al.Single injections of a DNA plasmid that contains the human bcl-2 gene prevent loss and atrophy of distinct neuronal populations after apinal cord injury in adult rats[J].Neuroehabil Neural Repair,2000,14(4):319-330.
  • 9Coll D E,Thompson V F,Li H Q,et al.The calpain system[J].Physiol Rev,2003,83(3):731-801.
  • 10刘同慎,刘希琴,白咸勇,成令忠.Bax蛋白在大鼠肝癌发生过程中的表达和意义[J].中国组织化学与细胞化学杂志,2000,9(3):269-271. 被引量:4

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