摘要
为探讨X射线辐射对仔鼠肝重量、肝组织结构及肝中Bax蛋白表达的影响,对160只仔鼠(出生6—7d)。用不同剂量(0、1、3、5、7Gy)的X射线进行全身辐射,分别于辐射后1、5、10、20d,检测各期仔鼠肝重和体重的变化,用显微技术观察仔鼠肝脏组织结构的变化,用免疫组织化学方法检测肝脏中Bax蛋白的表达。结果表明,X射线辐射影响仔鼠肝脏的生长发育。辐射后5—20d,辐射组仔鼠肝重均低于对照组。差异显著(P〈0.05)或极显著(P〈0.01):除1Gy辐射组外,其他辐射组在5-20d时仔鼠体重均低于对照组.差异极显著(P〈0.01);随辐射剂量增大,辐射组仔鼠肝中Bax蛋白阳性表达逐渐增强;辐射后仔鼠肝小叶结构不清,肝板变细,肝血窦变大,肝细胞变形,排列不规则。细胞核萎缩,细胞出现空泡化。
To explore the effects of X-ray on weight, histological structure and expression of Bax protein in liver of developing filial mice, 160 mice of 6-7 days after birth were radiated with different dosages (0, 1, 3, 5, 7Gy) of X-ray. Changes of liver and body weight were examined, histological structure was observed using bio-microscopy,and the expression of Bax protein was measured by immunohistochemical staining in filial mice liver at 1, 5, 10 and 20d after irradiation. Results show that X-ray influences the growth and development of the filial mice liver. The liver weight of filial mice of the experimental groups is lower than that of the control group 5-20d after irradiation (P〈0.05 or P〈0.01), except experimental groups with 1Gy; the body weight of filial mice of the experimental groups is lower than that of the control group 5-20d after irradiation (P〈0.01); the expression intensity of Bax protein in the liver of filial mice is significantly increased with the increase of the dose; the structure of hepatic lobule is unclear, hepatic plate is thinner, hepatic sinusoid is stronger, accompanied with hepatocyte disfiguration, irregular arrangement, nucleus atrophy, and vacuolization, after irradiation.
出处
《科技导报》
CAS
CSCD
北大核心
2010年第13期87-91,共5页
Science & Technology Review
基金
国家自然科学基金项目(30370163)
甘肃省创新人才基金项目(ZS006-016)
关键词
X射线
仔鼠
肝
组织结构
BAX蛋白
X-ray
filial mice
liver
histological structure
Bax protein