摘要
提取螺旋藻多糖的传统工艺存在着流程长,操作复杂,有机溶剂消耗大等问题。采用三氯乙酸(TCA)法代替Sevag法去除蛋白质,使改进后的提取工艺中蛋白质去除率增加,多糖损失率降低,流程缩短。通过用DEAE-Speharose和Sephadex-G50柱的分离纯化得到2个多糖组分。经测定,其相对分子质量分别为Mr1=12600,Mr2=16600。组分1的单糖组成:D-葡萄糖、D-甘露糖、D-半乳糖和葡萄糖醛酸;组分2的单糖组成:D-葡萄糖、D-木糖、L-鼠李糖和葡萄糖醛酸。
New process was
proposed to improve the traditional process. Protein removal ratio , polysaccharide yield,
operation time, the amount of organic solution, etc, were compared in this paper. The TCA
method, used to remove protein instead of Sevag method , not only reduces the loss of
polysaccharide but also increase the removal ratio of the protein. The operation time of the new
process was 5 h short comparing with the traditional one. Purified by DEAESepharose and
SephadexG50 column, two polysaccharide fractions were obtained. The molecular weight of
these two fractions were 12 600 and 16 000, and the composition of fraction 1 is Dglucose,
Dmannose, Dgalactose, glucuronic acid, fraction 2 is Dglucose, Dxylose, Lrhommose and
glucuronic acid respectively.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
1999年第2期15-18,共4页
Food and Fermentation Industries
关键词
螺旋藻多糖
纯化
提取
多糖
Spirulina platensis, polysaccharide, separation and
purification