固定化细胞酶法拆分N-乙酰-D,L-3-甲氧基丙氨酸

Resolution of N-Acetyl-D,L-3-methoxy-alanine by Immobilized Cells with Aminoacylase

利用氨基酰化酶固定化细胞酶法拆分了N-乙酰-D,L-3-甲氧基丙氨酸.考察了温度、pH值、底物浓度、金属离子和拆分时间对酶促反应的影响.确定了氨基酰化酶固定化细胞手性拆分N-乙酰-D,L-3-甲氧基丙氨酸的最佳工艺条件为pH=7.0、反应温度50℃及底物浓度500mmol/L.10-4mol/L的Co2+和Mg2+对氨基酰化酶有显著激活作用,Cu2+和Zn2+对酶促反应有明显抑制作用.在最佳条件下,氨基酰化酶固定化细胞对N-乙酰-L-3-甲氧基丙氨酸的摩尔转化率达96%.

As a pharmaceutical intermediate,L-serine plays an important role in organic synthesis and peptide synthesis.Also N-acetyl-D,L-3-methoxy-alanine is an intermediate in synthesis of L-serine from methyl α-bromo-β-methoxypropionate.In this thesis,a new method of resolution of N-acetyl-D,L-3-methoxy-alanine by immobilized cells with aminoacylase was studied.Several factors of this enzymatic reaction were investigated.The optimal temperature and pH are 50 ℃ and 7.0.In 10 mL reaction solution,the optimal concentration of N-acetyl-D,L-3-methoxy-alanine is 500 mmol/L and the amount of immobilized cells is 1 g.10^-4 mol/L of Co^2＋ and Mg^2＋ are benefit to aminoacylase,while Cu^2＋ and Zn^2＋ show clearly inhibitory effect.Under the optimal conditions,molar conversion rate of N-acetyl-L-3-methoxy-alanine is 96%.