摘要
目的:研究黄连解毒汤有效部位(HLJDTAF)对神经细胞内钙超载的影响及相关机制。方法:低糖低氧损伤胎鼠皮层神经细胞模拟脑缺血,双波长荧光分光光度法测定神经细胞内钙含量,观察HLJDTAF对细胞内钙超载的抑制作用。并分别采用谷氨酸(Glu),KCl,A23187,乙酰甲胆碱(MCh)和咖啡因(CAF)处理神经细胞,以分析药物的作用环节。结果:HLJD-TAF对低糖低氧处理所致神经细胞钙超载有明显的抑制作用(P<0.05);对200μmol.L-1谷氨酸,50 mmol.L-1剂量的K+,0.05μmol.L-1钙离子载体A23187处理细胞引起神经细胞内钙超载有明显的抑制作用(P<0.05,P<0.01);正常外钙情况下和零外钙情况下,HLJDTAF对5 mmol.L-1CAF和Mch诱导神经细胞内钙超载有明显的抑制作用(P<0.01,P<0.05)。结论:HLJDTAF可显著抑制神经细胞拟缺血损伤后的钙超载,其机制可能是通过多途径抑制缺血缺氧后神经细胞内钙超载,从而拮抗脑缺血损伤。
Objective: To investigate the effect and mechanism of Huanglian Jiedu Tang active fraction(HLJDTAF) on calcium overloading in neurons.Method: Cerebral ischemia was imitated by hypoxia/hypoglycemia damage on fetal rat neurons.Double wavelength fluorospectrophotometry was used to assay the content of calcium in neurons in order to evaluate the effect of HLJDTAF on calcium overloading.Neurons were treated with glutamic acid,potassium chloride(KCl),A23187,caffeine(CAF) and methacholine(Mch) to analysis the related mechanism of HLJDTAF on calcium overloading in neurons.Result: HLJDTAF 0.3,0.15 g·k-1 could remarkably inhibit the calcium overloading in neurons caused by hypoxia/hypoglycemia,glutamic acid,KCl and A23187.HLJDTAF 0.3 g·kg-1could inhibit the increasing of calcium caused by CAF and Mch in the presence of and in the absence of extra-calcium.Conclusion: HLJDTAF could remarkably inhibit the calcium overloading in neurons after cerebral ischemia injury,it probably plays the function via several pathways.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第16期2166-2170,共5页
China Journal of Chinese Materia Medica
关键词
黄连解毒汤有效部位
脑缺血
钙超载
神经细胞
Huanglian Jiedu Tang active fraction(HLJDTAF)
cerebral ischemia
intracellular calcium
neuron