摘要
目的研究成纤维细胞生长因子受体2(FGFR2)基因rs2981579单核苷酸多态性与中国汉族女性乳腺癌患病风险及临床病理特征的关系。方法抽取321例汉族女性乳腺癌患者和330名健康女性对照组外周血,抽提基因组DNA,采用聚合酶链反应连接酶检测反应(PCR-LDR)检测FGFR2 rs2981579基因多态性,比较基因型分布和患病风险及临床病理特征的关系。结果 rs2981579低频等位基因频率在病例组和对照组分别为46.9%和46.3%。以CC基因型为参照,CT或TT基因型没有显著提高乳腺癌的患病风险,其中携带CT基因型患病风险为OR=0.972,95%CI:0.648~1.458;携带TT基因型患病风险为OR=1.042,95%CI:0.668~1.626。rs2981579单核苷酸多态性与发病年龄、淋巴结转移、雌孕激素受体状态等临床病理参数无相关性。结论 FGFR2基因rs2981579多态性不是中国汉族女性乳腺癌患病风险因子和预后预测因子。
Objective To evaluate the association between the rs2981579 polymorphisms of fibroblast growth factor receptor 2(FGFR2) and breast cancer risk in a hospital-based Chinese population.Methods Genomic DNA was extracted from peripheral blood lymphocytes.Polymerase chain reactionligation detection reaction(PCR-LDR) was used to genotype in 321 breast cancer cases and the 330 controls.Results The minor allele gene frequencies of 2981579 in the patients and in the controls were 46.9% and 46.3% respectively.CT genotype OR = 0.972,95% CI:0.648 ~ 1.458 and TT genotype OR = 0.726,95% CI:0.431 ~ 1.225 did not confer a significantly increased risk for breast cancer,compared with the CC genotype.No significant correlation was noted between the FGFR2 rs2981579 and lymph node metastases,estrogen receptor status or progesterone receptor status.Conclusion These observations suggests that FGFR2 rs2981579 polymorphisms may not play an important role as genetic markers for breast cancer risk and may not the useful for tumor prognosis in Chinese women.
出处
《苏州大学学报(医学版)》
CAS
北大核心
2010年第3期528-531,共4页
Suzhou University Journal of Medical Science
基金
无锡市科技局科技发展计划资助项目(CAE00801-08)
无锡市科技局社会发展计划资助项目(CSE00708)