摘要
对八仙花组织培养的初代培养、继代培养过程做了初步研究和探讨。以八仙花的茎段、茎尖、叶片为外植体,通过MS+6-BA2.0 mg.L-1+IBA0.1 mg.L-1培育出无菌苗。继代培养在MS培养基中加入不同浓度的6-BA(6-苄基腺嘌呤)和IBA(吲哚丁酸)。试验结果表明:在初代培养中的外植体以八仙花茎尖较为适宜,消毒时间7 m in较为适宜。继代培养中以MS+6-BA1.0 mg.L-1+IBA0.1 mg.L-1为八仙花较适宜的增殖培养基。
The process of initial culture subculture of Hydrangea macrophylla culture were studied discussed.Taking stems,shoot tip,leaf of Hydrangea macrophylla as explants,aseptic seedlings were cultivated by MS +6-BA2.0 mg · L-1 + IBA0.1 mg· L-1.Subculture is to add different concentrations of 6-BA(6-benzyl adenine) IBA(indole butyric acid) in MS medium.Result shows that: shoot tips of Hydrangea macrophylla is optimum in initial culture of explants,and the optimum disinfection time is 7 min.The optimum proliferation medium for Hydrangea macrophylla is MS +6-BA1.0 mg· L-1 + IBA0.1 mg · L-1 in subculture.
出处
《防护林科技》
2010年第5期48-49,55,共3页
Protection Forest Science and Technology
关键词
组织培养
八仙花
不定芽
tissue culture
Hydrangea macrophylla
adventitious buds