绿色荧光蛋白基因标记芒果畸形病病原菌研究

Transformation of Fusarium proliferatum with the green fluorescent protein gene

芒果畸形病是世界性的芒果重要病害。采用PEG介导的原生质体转化法用绿色荧光蛋白(GFP)表达载体(pCT74-sGFP)转化芒果畸形病病原菌(Fusarium proliferatum),获得了表达GFP的转化子。转化子经过单孢纯化后连续继代培养仍能发出稳定而强烈的荧光,通过GFP特异性引物PCR扩增转化子基因组获得预期片段,表明GFP基因已成功转入芒果畸形病病原菌基因组中且稳定遗传。GFP标记菌株生长正常,致病性和野生菌丝无差别。获得GFP标记的转化子,为应用发绿色荧光的芒果畸形病病原菌研究病原菌的侵染方式、扩展过程等奠定了基础。

Fusarium proliferatum was the causal agent of malformation of mango in China,a persistent and economically important disease in worldwide mango growing areas.The paper described the transformation of this fungus with the construct （pCT74） containing the green fluorescent protein （GFP）.Polyethylene Glycol （PEG） mediated transformation of protoplast of the fungus yielded mitotically stable transformants with no change in virulence on mango plants in comparison to the wild type.These transformants would be critical tools for elucidating fungal penetration mode of host plants,invasive growth,and the nature of its host association.