三种化合物对石棉致HEL细胞增殖周期及c-fos癌基因转录活性改变的影响

Effects of Three Kinds of Chemicals on the Changes in Chrysotile induced Cell Proliferation Cycles and c fos Oncogene Transcription Activity in Human Embryo Lung Cells

目的寻求一种适宜的石棉表面改性剂。方法利用流式细胞技术测定细胞周期以及Ｎｏｒｔｈｅｒｎ杂交技术测定ｃｆｏｓ癌基因转录活性，观察几种化合物对温石棉促人胚肺（ＨＥＬ）细胞增殖作用的影响。结果将ＨＥＬ细胞与温石棉共同孵育后，其Ｓ期细胞所占比例增加，ｃｆｏｓ癌基因的转录活性也明显增强。用３种化合物预处理的石棉组，其Ｓ期细胞所占比例以及ｃｆｏｓ癌基因的转录活性均低于未处理石棉组。结论用上述３种化合物预处理石棉，均可不同程度地抑制石棉的促细胞增殖作用。

Objective To seek for an ideal chemical for surface modification of asbestos.Methods Cell cycles were measured with flow cytometry and activity of c fos oncogene transcription was determined by Northern blotting technique to observe the effects of several kinds of chemicals on cell proliferation cycles in human embryo lung (HEL) cells induced by chrysotile.Results Proportion of HEL cells in the S phase,as well as activity of transcription of c fos oncogene,increased obviously after incubation with chrysotile.Proportion of HEL cells in the S phase and activity of transcription of c fos oncogene reduced after pretreatment with three kinds of chemicals,as compared to those before pretreatment.Conclusion Pretreatment with the three kinds of chemicals could inhibit proliferation promoting effect of chrysolite,to various extent.