水仙离体培养及植株再生的研究进展

Advances on Narcissus in vitro Culture and Plant Regeneration

水仙离体培养及其再生技术在水仙品种改良、种质资源保存与繁殖、遗传转化等方面发挥着重要作用。本文综述了20世纪70年代以来水仙离体培养及植株再生的研究进展,包括水仙营养器官培养、生殖器官培养、体细胞培养等方面的应用,对水仙离体培养过程中存在的外植体依赖性较强、悬浮细胞振荡培养研究、体细胞培养研究等主要问题进行了初步探讨,并对今后水仙离体培养的外植体选取、新的离体培养途径等研究方向作了展望。

The techniques of in vitro culture and Regeneration forNarcissus plants can be applied toNarcissus variety improvement, preservation and propagation of germplasm and genetic transformation. In this paper, the recent progress on research ofNarcissus in vitro culture was reviewed including vegetative organ culture, reproductive organ culture and somatic cell culture. Some primary problems at present in this field were preliminarily discussed including strong explants dependencies, suspension cell culture research and somatic cell culture research et al., and future research works such as selecting the appropriate explants and in vitro culture new ways were put forward.