3个基因SNPs及基因聚合对白耳鸡产蛋数的遗传效应

Effect of Single and Pyramiding Genotypes of Three Genes on Egg Production of Baier Chicken

将GH、POU1F1和PRL基因作为影响鸡繁殖性状的候选基因,以白耳鸡为试验材料,采用PCR-SSCP方法检测白耳鸡GH、POU1F1和PRL基因单核苷酸多态性,分析这3个基因的单基因以及聚合基因型对白耳鸡72周龄产蛋数影响。结果表明:GH、POU1F1和PRL基因均对白耳鸡72周龄产蛋数的差异产生影响,与鸡的产蛋数存在关联,AA、CC、EE型为优势单基因型;优势单基因型两两聚合的遗传效应高于优势单基因型的遗传效应;3个优势单基因型聚合(AACCEE型)遗传效应又高于优势单基因型两两聚合的遗传效应,因此我们推断可能聚合的优势单基因型越多,对白耳鸡72周龄的产蛋数影响效应越大,所以可以尝试利用聚合基因型对白耳鸡的72周龄产蛋数进行标记辅助选择。

The chicken GH,POU1F1 and PRL genes were selected as candidate genes,PCR-SSCP method was used to identify the polymorphism sites in the three genes.This study was designed to investigate the effect of GH,POU1F1 and PRL genes on egg production of 72-week-old Baier chickens,the single genotype effects as well as the pyramiding genotype effects were all studied.The results showed that GH,POU1F1 and PRL genes had effect on egg production of 72-week-old Baier chickens,they were candidate loci or linked to major genes that affected egg production of 72-week-old chickens;AA,CC and EE were three advantageous single genotypes.The effects of two advantageous genotypes pyramiding genotypes were higher than that of the single genotype.The effects of three advantageous pyramiding genotypes （AACCEE） were higher than that of two advantageous pyramiding genotypes.Furthermore,the more loci,the larger effects on egg production of 72-week-old chickens,It may be concluded that pyramiding genotypes are superior to single genotype as potential molecular markers for egg production in chickens.