淫羊藿苷对骨质疏松模型小鼠骨组织中IL-6表达的影响

Effects of icariin of examining the expression level of interleukin-6 in mice models of experimental osteoporosis

目的观察淫羊藿苷对骨质疏松模型小鼠骨组织中白介素-6(IL-6)表达的影响,探讨淫羊藿苷的抗骨质疏松作用机制。方法取雌性小鼠于无菌条件下切除双侧卵巢,复制骨质疏松小鼠模型。造模小鼠随机分为:模型,己稀雌酚、淫羊藿苷高、中、低剂量组,以假手术小鼠作为对照组。各组动物按各自药物和剂量灌胃给药,每日1次,连续2个月。停药次日,脱颈椎处死动物,取右侧股骨常规脱钙、石蜡包埋、切片,用免疫组化方法观察骨组织白介素-6(IL-6)的表达。结果与对照组比较,模型组小鼠骨组织中IL-6阳性破骨细胞灰度值明显降低;与模型组比较,淫羊藿苷高、中剂量组小鼠骨组织中IL-6阳性破骨细胞灰度值明显增加。结论淫羊藿苷通过降低骨质疏松模型小鼠骨组织中IL-6的表达,从而减少骨吸收。

Objective To investigate the effects of Icariin of examining the expression level of interleukin-6 （ IL-6） in mice models of experimental osteoporosis,and to explore the mechanism of Icariin.Methods Reproduce the osteoporosis model of mice with ovarian removal of female mice.All mice were divided into six groups which were model group,positive drug group,Icariin high dose group,Icariin middle dose group,Icariin low dose group,and sham operation group.Each animal group according to their drug dosage was given oral administration once a day.After two months,the mice were sacrificed.Take the right femur routine decalcified,embedded in paraffin,sliced,the levels of interleukin-6 in bone tissue were determined by using immunohistochemistry techniques.Results As compared with Sham group,the positive gray value of IL-6 of Model group was significantly reduced.As compared with model group,the positive gray value of IL-6 was larger in Icariin high dose group and Icariin middle dose group.Conclusion Icariin can reduce the absorption the bone by reducing IL-6 expression in bone tissue.