CD54和HLA ABC在肝癌、肝硬化组织中的表达

Expression of CD54 and HLA ABC on hepatocellular carcinoma and liver cirrhosis

目的：研究ＣＤ５４ 及ＨＬＡＡＢＣ 在肝细胞肝癌（ ＨＣＣ） 及肝硬化（ＬＣ） 组织中的表达、定位及其意义。方法：以免疫组化ＡＰＡ 法，检测ＣＤ５４ 和ＨＬＡＡＢＣ 在ＨＣＣ 及ＬＣ 组织中的表达和定位。结果：ＣＤ５４ 在ＨＣＣ 组织中的阳性率为７７ ．１ ％ （３７／ ４８） ， 且以膜表达为主， 呈蜂窝样排列； 在ＬＣ 组织中的阳性率为４０ ．０ ％ （１２／ ３０） ， 以核表达为主。ＨＬＡＡＢＣ 在ＨＣＣ 组织中的阳性率为３０ ．０ ％ （１２／４０） ，在ＬＣ 组织中的阳性率为６３ ．３ ％ （１９／３０） 。ｘ２ 检验，ＨＣＣ 组织中ＣＤ５４ 的表达高于ＬＣ，两者相差显著（ Ｐ＜ ０ ．０５） ，Ｐｏ ｗｅｒ ＝ ０ ．９２ 。ＨＣＣ 组织中ＨＬＡＡＢＣ 的表达低于ＬＣ，两者相差显著（ Ｐ ＜ ０ ．０５） ，Ｐｏｗｅｒ ＝ ０ ．８０ 。结论：ＣＤ５４ 在ＨＣＣ 组织中的高度表达并不能引起有效抗肿瘤免疫应答，原因之一可能是ＨＬＡＡＢＣ 在ＨＣＣ 组织中的表达率降低所致。

Aim: To study the expression, distribution and significance of CD54 and HLA ABC in liver cirrhosis(LC) and hepatocellular carcinoma(HCC) tissues. Methods: CD54 and HLA ABC were detected in HCC and LC tissues by immunohistochemical methods. Results: The positive rate of CD54 in human HCC tissues was 77.1%(37/48). In most cases(25/48) it was expressed on the HCC cell surface with honeycomb like appearance. In LC tissues, the positive rate of staining against CD54 was 40.0%(12/30) with the majority showing nuclear staining. The positive rate of HLA ABC was 30.0%(12/40) in HCC tissues, but in LC tissues, it was 63.3%(19/30).Statistical analysis suggested that the expression of CD54 was significantly higher in HCC tissues than that in LC tissues (P < 0.05, Power=0.92). HLA ABC expression was significantly lower in HCC tissues than that in LC tissues (P < 0.05, Power=0.80). Conclusion: In HCC tissues, the higher expression of CD54 could not evoke effective anti tumor immune response, one of its reasons may be the lower expression of HLA ABC.