奥替普拉诱发人肺腺癌细胞凋亡的研究

Studies on Apoptosis of Human Lung Adenocarcinoma Cells Induced by Oltipraz

目的探讨奥替普拉（ｏｌｔｉｐｒａｚ）能否诱发人肺腺癌细胞凋亡及其机制。方法应用光镜、ＤＮＡ电泳、流式细胞仪、活细胞视频观察进行检测和分析。结果癌细胞在奥替普拉作用下，细胞分裂阻滞在分裂中期，Ｇ２／Ｍ百分比增高，出现细胞凋亡峰，凋亡细胞出现胞体固缩、核染色质凝聚或者断裂，细胞ＤＮＡ裂解片段呈现典型的“梯形”条带。奥替普拉诱导的ＧＬＣ细胞凋亡在使用浓度１２０μｇ／ｍｌ时效果明显。结论奥替普拉在适当浓度时能够诱发人肺腺癌细胞凋亡，这种作用与分裂期阻滞密切相关。

Objective To study whether oltipraz can induce apoptosis of human lung adenocarcinoma (GLC 82) cells and its possible mechanism. Methods Light microscopy, DNA electrophoresis, flow cytometry and video time lapse monitoring were used to observe apoptosis of GLC 82 induced by oltipraz. Results GLC 82 cells treated with oltipraz underwent the arrest of cell mitosis at metaphase, higher percentage of G 2/M and peak of apoptosis were found, and cell body shrunk, nuclear chromosome condensed or fragmented and nuclear DNA fragment displayed “laden” bands in apoptic cells. Apoptosis of GLC 82 induced by oltipraz was more obvious at a concentration of 120 μg/ml. Conclusion Oltipraz could induce apoptosis of GLC 82 at certain concentrations, which associated closely with the arrest of mitotic cycle.