基质辅助激光解吸电离飞行时间质谱技术用于常见益生菌鉴定的初步研究

Primary research on identification of common probiotics by MALDI-TOF mass spectrometry

目的建立基质辅助激光解吸电离飞行时间质谱(MADLI-TOF MS)技术鉴定常见益生菌的实验方法并对MADLI-TOF MS技术的适用性进行初步评价。方法对MADLI-TOF MS技术鉴定常见益生菌过程中各影响因素进行考察,筛选出最佳的实验条件。利用19株供试菌株所得的蛋白指纹图谱对MADLI-TOF MS技术的适用性进行研究。结果建立了MADLI-TOF MS技术鉴定常见益生菌的最佳实验方法。初步证明MADLI-TOF MS技术具备在属、种、亚种以及菌株水平上鉴定常见益生菌的能力。结论建立的实验方法稳定性高、重复性好,可以作为MADLI-TOF MS技术鉴定常见益生菌的参考方法。MADLI-TOF MS技术可以作为常见益生菌鉴定的方法之一。

Objective To establish the experimental method for identification of common probiotics by matrix-assisted laser desorption/ionization time of flight（MALDI-TOF） mass spectrometry,and evaluate the applicability of MALDI-TOF mass spectrometry.Method Optimal experimental conditions were determined through studying the influence factors during the identification by MALDI-TOF mass spectrometry.The applicability of MALDI-TOF mass spectrometry was evaluated by protein fingerprinting of 19 experimental strains.Result Optimal experimental method for identification common probiotics by MALDI-TOF mass spectrometry was established.MALDI-TOF mass spectrometry was able to identify common probiotics at genus,species,subspecies and strain levels.Conclusion The established experimental method,which has high stability and good reproducibility,may be used as a standardized protocol.MALDI-TOF mass spectrometry can be used as one of the methods for common probiotics identification.