荷兰水仙组织培养再生体系关键技术研究

Study on Subculture of Narcissus L. cv. Delibes

利用双鳞片法进行荷兰水仙组织培养,研究继代培养过程中影响出芽、小鳞茎形成和生根等的生长因子,并用多效唑(PP333)处理组培材料,研究PP333对继代培养材料生长的影响。结果表明,MS培养基中添加6-BA1.0mg·L-1、蔗糖15.0g·L-1时出芽效果最好;MS培养基中添加6-BA4.0mg·L-1、NAA0.2mg·L-1、活性炭2.0mg·L-1、蔗糖60.0g·L-1时或MS培养基中添加6-BA2.0mg·L-1、2,4-D1.0mg·L-1、活性炭2.0mg·L-1、蔗糖90.0g·L-1时小鳞茎形成效果最好;MS培养基中添加6-BA1.0mg·L-1、2,4-D0.5mg·L-1、NAA0.5mg·L-1、活性炭2.0mg·L-1、蔗糖30.0g·L-1时生根效果最好。最适合荷兰水仙组培继代培养材料矮壮的PP333浓度为0.5mg·L-1。该研究可为荷兰水仙种苗工厂化生产提供技术支持。

In vitro-grown shoots were obtained from bulb twin scales of Narcissus. Shoot induction, bulbil formation and root differentiation on subculture were studied. The effects of 6-BA, 2,4-D, NAA, activated charcoal and sucrose were evaluated by the orthogo- nal design [L16（45）]. The results showed that the optimum medium for shoot induction was MS medium supplemented with 6-BA 1.0 mg·L-1 and sucrose 15.0 g·L-1; The optimum medium for bulbil formation was MS medium supplemented with 6-BA 4.0 mg·L-1, I NAA 0.2 mg·L-1, activated charcoal 2.0 mg·L-1 and sucrose 60.0 mg·L-1 or MS medium supplemented with 6-BA 2.0 mg·L-1, 2,4-D 1.0 mg·L-1, activated charcoal 2.0 mg·L-1 and sucrose 90.0 g·L-1 Roots could be induced in MS medium supplemented with 6-BA 1.0 mg·L-1, 2,4-D 0.5mg·L-1, NAA 0.5 mg·L-1, activated charcoal 2.0mg·L-1 and sucrose 30.0 g·L-1. The most optimum concentration of PP333 for shoots dwarfing culture was 0.5 mg·L-1. This study provided efficient micropropagation protocol of Narcissus.