东北红豆杉愈伤组织中紫杉烷类化合物的分离与鉴定(2)

Isolation and identification of the taxoids from a callus culture of Taxus cuspidata(2)

目的进一步明确东北红豆杉（Taxus cuspidata）愈伤组织中的化学成分,为开发可持续利用的红豆杉资源提供物质基础。方法采用硅胶柱色谱、制备型高效液相色谱等手段对愈伤组织的甲醇提取物进行分离和纯化;根据化合物的理化性质和波谱数据并结合相关文献对化合物结构进行鉴定。结果从东北红豆杉愈伤组织甲醇提取物中分离得到7个紫杉烷型二萜类化合物,分别鉴定为taxusin（1）、2α-acetoxytaxusin（2）、14β-hydroxytaxusin（3）、2-deacetyltaxuyunnanine C（4）、10-deacetyltaxuyunnanine C（5）、7-表-紫杉醇（7-epi-taxol,6）、cephalomannine（7）。结论化合物1~5,7为首次从东北红豆杉愈伤组织中分离得到,化合物3~5尚未在东北红豆杉中发现。

Objective To investigate the chemical constituents of the callus culture of Taxus cuspidata.Methods The compounds were isolated and purified from ethyl acetate and methanol extracts of the callus culture by silica gel column chromatography and preparative HPLC,and the structure elucidation was carried out by analyses of the physico-chemical constants and the spectral data.Results Seven taxoids were obtained and identified as taxusin（1）,2α-acetoxytaxusin（2）,14β-hydroxytaxusin（3）,2-deacetyltaxuyunnanine C（4）,10-deacetyltaxuyunnanine C（5）,7-epi-taxol（6）and cephalomannine（7）,respectively.Conclusions Compounds 15 and 7 are reported from the callus culture of T.cuspidata for the first time,and compounds 35 are not found yet in the plant of this species.