聚合酶链反应检测新生儿衣原体感染

DETECTION OF CHLAMYDIAL INFECTION IN NEWBORN BY POLYMERASE CHAIN REACTION

目的用聚合酶链反应检测新生儿衣原体感染状况。方法参照文献报道的沙眼衣原体及肺炎衣原体基因序列，分别设计一对引物，采用ＰＣＲ法检测住院新生儿衣原体感染。结果因呼吸道感染住院的１２３名新生儿的咽拭子标本，沙眼衣原体及肺炎衣原体感染率分别为１３．８％和４．９％。结论ＰＣＲ检测沙眼衣原体及肺炎衣原体感染具有特异、敏感、快速的特点，可为临床诊断提供科学依据。

Aim Detection of chlamydial infectious condition in newborn with polymerase chain reaction Methods A pair of primers each were synthesized according to genic sequences of chlamydia trachomatis and chlamydia pneumoniae in the references The chlamydial infections in newborn were detected with PCR Results Ct and Cp positive rates were 13 8% and 4 9% respectively in the nasopharyngeal secretion samples collected from 123 hospitalized newborn with different diseases Conclusions PCR in a sensitive,rapid and specific method for diagnosis of chlamydial infection