麻杏口服液中绿原酸含量测定方法的建立

Establishment of Method for Content Determination of Chlorogenic Acid in Maxin Oral Liquid

目的:建立麻杏口服液中绿原酸的含量测定方法。方法:采用高效液相色谱法,色谱柱为Kromasil 100-5 C18(4.6mm×250 mm,5μm),流动相为乙腈-0.1%磷酸(10∶90),流速为1 mL·min-1,柱温为30℃,检测波长327 nm;结果:绿原酸在15.84～95.04 mg·L-1线性关系良好,标准曲线方程为Y=27.606X+27.927,r=0.999 5;结论:该方法稳定可行,结果准确,可用于麻杏口服液的质量控制。

Objective： To set up the method for content determination of chlorogenic acid of Maxin oral.Method： HPLC was adopted performing on a kromasil C18 column（4.6 mm × 250 mm,5 μm） with acetonitrile-0.1%（w） phosphoric acid（10∶ 90） as the mobile phase at a flow rate of 1.0 mL.min-1.The detective wave length was set at 327 nm and the column temperature was at 30℃.Result：Chlorogenic acid was linear in the range of 15.84-95.04 mg·L-1.The standard curve equation was Y = 27.606 X ＋ 27.927,r = 0.999 5.Conclusion： The method set up was stable,feasible and accurate,and it can be used to control the quality of Maxin oral.