水稻与病原物互作蛋白质组学研究进展

Recent Advances in Proteomic Studies on Rice-Pathogen Interactions

植物与病原物互作的分子机制一直是植物病理学研究的热点。近年来,功能基因组学研究策略,包括蛋白质组学和转录组学,已广泛应用于基因、蛋白的功能鉴定和表达谱研究。蛋白质组学作为转录组学研究的一个互补内容,已成为系统研究植物应答病原物侵染的蛋白质表达谱和翻译后修饰的有力工具。水稻作为已完成基因组测序的单子叶植物,是研究植物与病原物互作分子机理的理想模型。因此,着重介绍了蛋白质组学的研究技术,水稻响应细菌、真菌及病毒等病原物侵染的蛋白质组学研究进展和水稻与病原物互作磷酸化蛋白质组学的概况。

The molecular mechanisms of plant-pathogen interactions have been a longtime hotspot of plant pathology.Recently,functional genomic strategies,including proteomics and transcriptomics,have been widely used in defining gene and protein function and expression profiles.Proteomics,a complementary research content of transcriptomics,has been a powerful tool to systematically analyze the cellular protein expression profiles and post-translational modifications under pathogen invasion.Rice,a monocotyledonous plant for which the entire genome was completely sequenced,has been adopted as an ideal model for studying the molecular mechanisms of plant-pathogen interactions.In this review,the main research techniques of proteomics,recent advances in proteomic analyses of rice under bacteria,fungi and viruses invasion were highlighted,and an overview on phosphoproteomics analyses of rice-pathogen interactions was presented.