RKIP与NF-κB在肺癌侵袭转移中的作用

Role of Raf Kinase Inhibitor Protein and Nuclear Factor-κB in the Invasion and Metastasis of Lung Carcinoma

目的:检测肺癌组织中Raf激酶抑制蛋白(raf kinascinhibitor protein,RKIP)和核因子κB(nuclearfactor-κB,NF-κB)的表达,分析其与临床病理特征及预后的关系。方法:应用免疫组织化学方法(SP法)检测83例肺癌及65例癌旁正常肺组织中RKIP与NF-κB蛋白的表达情况。结果:83例肺癌中,RKIP蛋白的低表达率及NF-κB蛋白的高表达率分别为20.5%(17/83)、68.7%(57/83),其表达水平与肺癌的分化程度、TNM分期、淋巴结或远处转移及术后生存时间有明显关系(P均<0.05);RKIP蛋白的表达水平与NF-κB呈负相关(P<0.05);RKIP蛋白低表达与NF-κB蛋白高表达的病例平均生存时间和5年生存率明显降低(P<0.05)。结论:RKIP低表达与肺癌的侵袭、转移密切相关,RKIP可能是肺癌的一个转移抑制蛋白,RKIP表达下调可能通过活化NF-κB信号通路促进肺癌细胞的侵袭和转移。

Objective： To investigate the expression of raf kinase inhibitor protein （RKIP） and NF-KB in lung carcinoma and its correlation with clinicopathologic features and survival time. Methods： RKIP and NF-KB protein levels were detected using immunohistochemistry in 83 lung carcinoma specimens and 65 non-tumor lung samples. Results： Immunohistochemistry revealed that of the 83 carcinoma cases, there was low expression of RKIP and high expression of NF-KB protein, 20.5% （17/83） and 68.7% （57/83）, respectively. Expression levels of RKIP and NF-KB protein were correlated with tumor differentiation, TNM staging, lymph node metastasis, and survival time （P〈0.05, P〈0.05, P〈0.05, P〈0.05, respectively）. RKIP expression was negatively correlated with NF-KB expression in lung carcinoma （P〈0.05）. The mean survival time in cases with a low expression level of RKIP protein was significantly shorter than those with a high expression level, whereas the mean survival time in cases with a high expression level of NF-KB protein was significantly shorter than those with a low expression level （P〈0.05）. Conclusion： Expression of RKIP and NF-KB are closely correlated with invasion and metastasis of lung carcinoma. RKIP may be a lung carcinoma metastasis suppressor, and decreased RKIP expression is associated with the increased metastatic potential of lung carcinoma cells through the activation of the NF-KB signaling pathway.