脐血血浆增强阿糖胞苷抗白血病效应的实验研究

Experimental study of cord blood plasma enhancing the antileukemia effect of AraC

目的研究脐血血浆（ＣＢＰ）对２８例急性髓系白血病（ＡＭＬ）细胞体外对阿糖胞苷（ＡｒａＣ）敏感性的影响。方法以基因重组人粒巨噬细胞集落刺激因子（ｒｈＧＭＣＳＦ）和空白组作对照，采用ＭＴＴ法检测ＡｒａＣ的细胞毒作用。结果ＡＭＬ细胞对ＡｒａＣ的敏感性表现出异质性，但使用ＣＢＰ及ｒｈＧＭＣＳＦ均可显著增强ＡｒａＣ对耐药ＡＭＬ细胞的毒性，与空白对照组相比，差异有显著性（Ｐ＜００５），尤以ＣＢＰ为优。结论ＣＢＰ与ｒｈＧＭＣＳＦ类似，可能通过促进耐药ＡＭＬ细胞进入增殖周期，从而增强ＡＭＬ细胞对ＡｒａＣ的敏感性，预示ＣＢＰ与ＡｒａＣ联合运用治疗耐药ＡＭＬ的潜在价值。

Objective To study the in vitro effect of cord blood plasma(CBP) on the sensitivity of acute myeloid leukemia(AML) cells to cytosine arabinoside (AraC). Methods Cytotoxicity of AraC was detected by MTT assay in twentyeight patients. Results and Conclusion The sensitivity of AML cells to AraC was heterogeneous,but CBP could significantly enhanced the cytotoxic activity of AraC to drug resistant AML cells.