nm23-H1基因杂合性缺失及突变与大肠癌转移抑制

THE RELATION OF THE LOSS OF HETEROZYGOSITY AND MUTATION FOR nm23 H1 WITH THE INHIBITION OF METASTASIS IN COLORECTAL CARCINOMA

应用Ｓｏｕｔｈｅｒｎ印迹杂交和ＲＴＰＣＲＳＳＣＰ银染、克隆及序列测定检测了３６例大肠癌组织标本中ｎｍ２３Ｈ１杂合性缺失和突变情况。结果：本组标本ｎｍ２３Ｈ１杂合性缺失率为２９．６３％，Ｄｕｋｅ’ｓＤ期及远处转移大肠癌有较高的杂合性缺失发生率；大肠癌中未见ｎｍ２３Ｈ１基因突变。本实验结果提示：ｎｍ２３Ｈ１基因杂合性缺失可能参与大肠癌恶性进展及转移过程的调节，该基因突变在大肠癌中所起的作用可能较小。

The loss of heterozygosity and mutation for nm23 H1 gene in colorectal carcinomas were studied by Southern blot and RT PCR SSCP/silver staining sequencing. The rate of loss of heterozygosity for nm23 H1 was 29.63%. The cases of Duke's stage D and distant metastatsis had higher frequency of the loss of heterozygosity. No mutation for nm23 H1 was found in colorectal carcinomas. These reaults indicate that the loss of heterozygosity for nm23 H1 may play a significant role in the malignant progression and distant metastasis in colorectal carcinomas.