Ⅰ型胶原蛋白多态性与脊柱融合的相关性分析

Relationship between the polymorphism of the collagen I alpha 1 gene and the vertebral fusion rate

目的研究Ⅰ型胶原蛋白(COLIA1)基因的多态性与脊柱融合患者术后融合效果的相关性。方法抽取需行脊柱融合患者外周静脉血3 mL,提取白细胞DNA并扩增目的基因所在的DNA片段;用PCR-RFLP检测外周血白细胞基因组CO-LIA1基因Pcol2位点多态性,扩增产物经限制性内切酶Eco31Ⅰ酶切,琼脂糖凝胶电泳观察;对照术前及术后3、6、12个月的X线片,观察植骨融合情况。结果两组实验对象均存在COL1A1基因Pcol2位点-1997G/T多态性,GG型166例,GT型232例,TT型25例。并发现GG基因型与颈椎融合有相关性(P=0.004),而与腰椎后路横突间植骨融合无相关性(P=0.831)。结论 COLIA1 Pcol2位点-1997G/T单核苷酸多态性GG基因型可能是促进颈椎前路自体髂骨植骨融合的重要遗传基因。

Objective To investigate the polymorphism of the colia1 and explore their relationship with the spinal fusion rate of the postoperative in Shandong Peninsula Area.Methods 3 mL peripheral blood was drawn-off and genomic DNA was extracted from white blood cells;The specific fragment which includes the target gene was amplified by Polymerase chain reaction（PCR）;the genotypes of Pcol2 site in COLIA1 were detected by Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）method.The PCR product was digested with restriction endonuclease Eco31Ⅰ and the result was observed by agarose gel electrophoresis.The G gene represents for the presence of the restriction endonuclease site,while the T gene for the absence of the restriction endonuclease site;To analyse the spinal fusion rate of the operational segment by comparing the x-ray film of preoperation and postoperation of 3,6,12 monthes.Results There were the-1997G/T polymophisms of the COLIA1 gene in the 423 subujects,the amount of the genotype GG is 166,GT 232 and TT 25.there is some correlation between the GG genotype and the C-spine auto-ilium graft fusion（P=0.004）.but the GG genotype has no correlation with the bone graft fusions inter transverse process of lumbar vertebra（P=0.831）.Conclusion The GG genotype in the-1997G/T polymophsim of the COLIA1 gene may be the essential factor which can promote the C-spine auto-ilium graft fusion.