甲胎蛋白异质体测定对原发性肝癌诊断的作用

Alpha-fetoprotein Heterogeneity on the Diagnosis of Primary Liver Cancer

目的探讨微量离心柱法测定甲胎蛋白异质体(AFP—L3)在良性肝病及原发性肝癌预警中的作用。方法以装有耦联小扁豆凝集素(LCA)的微量离心柱分离慢性肝炎组、肝硬化组、原发性肝癌组共52例的患者血清甲胎蛋白异质体,用化学发光方法检测分离前AFP和分离后AFP(AFP-L3),计算AFP-L3在AFP中的含量比例[AFP-L3(%)]。结果原发性肝癌组AFP-L3(%)均值、阳性率与慢性肝炎、肝硬化组相比均显著增加(P<0.001)。肝癌术后AFP-L3(%)含量比例显著下降(P<0.001)。结论微量离心柱法测定AFP-L3对肝癌和良性肝病的诊断具有重要意义。

Objective To evaluate the usefulness of new microspincolum method for the measurement of alpha-fetoprotein variant（AFP-L3） in early warming for benign liver disease and primary liver cancer.Methods The microspincolum coupled with lens culinaris agglutinin（LCA） was used to isolate the AFP-L3 from the serums of 52 patients with chronic hepatitis,cirrhosis of liver and primary liver cancer.AFP and AFP-L3 were determined by Electro-chemiluminescence Immunoassay Analyzer,and the proportion of AFP-L3 levels [AFP-L3（%）]were calculated.Results The positive rate and the mean level of AFP-L3 in the patients with primary liver cancer were statistic significantly different from the patients with chronic hepatitis or cirrhosis of liver（P0.001）.After received operation,the mean level of AFP-L3 were statistic significantly（P0.001）.Conclusion The microspincolum method detecting AFP-L3 shows important clinical value in the diagnosis of primary liver cancer.