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唾液链球菌嗜热亚种Y-2细胞转化法制备γ-氨基丁酸 被引量:5

Preparation of Gamma-Aminobutyric Acid Using Streptococcus salivarius subsp. thermophilus Y-2 Cells
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摘要 研究反应pH值、反应温度、重金属盐、表面活性剂、底物浓度、菌体质量浓度和磷酸吡哆醛添加量对Streptococcus salivarius subsp.thermophilus Y-2细胞转化法生产γ-氨基丁酸的影响。获得反应体系的最佳组成为:湿菌体25g/L、BaCl2 40mmol/L、Triton X-100体积分数0.02%、L-谷氨酸单钠盐(L-monosodium glutamate,MSG)47.5g/L和L-谷氨酸(L-glutamic acid,L-Glu)90.0g/L。该体系在40℃、pH 4.5和搅拌速度100r/min的最适转化条件下进行反应72h,转化液GABA产量达到了(87.16±4.33)g/L,细胞平均生产力为(48.42±2.41)mg/(h.g),摩尔转化率为(97.60±4.71)%。 The γ-aminobutyric acid(GABA) synthesis was conducted through biotransformation of Streptococcus salivarius subsp.thermophilus Y-2 cells to explore the effects of pH,reaction temperature,salts,surfactant,substrate concentration,strain concentration and addition of pyridoxal phosphate(PLP) on the production of GABA.Results indicated that the optimal reaction system included 25 g/L wet cells,40 mmol/L BaCl2,0.02% Triton X-100,47.5 g/L L-monosodium glutanate,and 90.0 g/L L-glutamic acid.The optimal reaction conditions of this reaction system were reaction temperature of 40 ℃,reaction pH of 4.5,stirring speed of 100 r/min and reaction time of 72 h.Under these optimal conditions,the yield of GABA was up to(87.16±4.33) g/L and biotransformation rate of GABA in moles was(97.60±4.71)%.The total productivity of the cells was(48.42±2.41) mg/(h·g).
出处 《食品科学》 EI CAS CSCD 北大核心 2011年第1期162-167,共6页 Food Science
基金 广东省自然科学基金项目(8452104101001546) 国家星火计划项目(2008GA780032) 韩山师范学院科研基金项目 国家"863"计划项目(2007AA10Z357)
关键词 唾液链球菌嗜热亚种 谷氨酸脱羧酶 Γ-氨基丁酸 生物转化 Streptococcus salivarius subsp.thermophilus glutamate decarboxylase γ-aminobutyric acid biotransformation
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