摘要
以火炬松×洪都拉斯加勒比松F1代群体为研究对象,从松树PGI(松类基因索引)数据中筛选出13个与赤霉素(GAs)代谢有关的序列。设计了这13条序列的EST-SSR引物对,并筛选出4对引物作为F1代检测的较好的标记。4对引物PCR分析显示在2个亲本和39个子代中共扩增出1014个多态性位点,其中,杂种F1代扩增出的位点数中有50.19%与父本相同,52.17%与母本相同,这表明母本(火炬松)和父本(加勒比松)杂交能够得到获得双亲遗传物质的新杂种。4个引物检测的26个等位基因位点中有6个与苗龄6个月的苗高有显著或极显著的相关性,有5个位点与苗龄9个月的苗高有显著或极显著相关性。这为早期选择提供了较好的分子标记。
Thirteen EST-SSR molecular markers related to GAs metabolism for Pinus L.were developed from EST sequences in PGI(Pinus gene index)database.1 014 alleles were amplified with two parents and 39 F1 progeny of Pinus taeda×P.caribaea var.hondurensis with four primer-pairs,which were selected from the thirteen EST-SSRs.50.19 % of the 1 014 alleles were originated from the male parent(P.taeda) and 52.17 % were shared with the female parent(P.caribaea var.hondurensis),which showed that the progeny of this hybridization inherent genetic materials from both parents.Six locus selected from all 26 locus amplified by four primer pairs were related significantly to six-month old seedling height of the 39 F1 hybrids,and five locus related significantly to nine-month old seedling height.Therefore,this markers can serve as good molecular markers for early selection.
出处
《南京林业大学学报(自然科学版)》
CAS
CSCD
北大核心
2011年第1期6-10,共5页
Journal of Nanjing Forestry University:Natural Sciences Edition
基金
浙江省科技计划项目(2008C32015)
国家农业科技成果转化资金项目(2009GB24320476)