摘要
目的:研究石榴皮总黄酮的提取工艺及黄酮类物质对亚硝化反应的抑制能力。方法:通过正交试验,探讨影响总黄酮提取率的因素范围和最佳组合,并采用分光光度法测定总黄酮提取液对亚硝酸盐清除能力和亚硝胺抑制能力。结果:石榴皮总黄酮的最佳提取工艺条件为乙醇体积分数60%、料液比1:60(g/mL)、提取时间40min、超声电流强度150mA。在最佳提取工艺条件下,石榴皮总黄酮的平均提取率为84.8mg/g,石榴皮总黄酮提取液对亚硝酸钠的最大清除率为81.2%,对亚硝胺合成的最大阻断率为67.0%。结论:石榴皮总黄酮提取液对亚硝化反应的抑制能力较强。
The optimal conditions for total flavonoids extraction from Punica granatum L.peel were studied by orthogonal array design and their abilities to scavenge nitrite and inhibit nitrosamines were tested spectrophotometrically.A maximum extraction yield of 84.8 mg flavonoids/g peel was obtained when a 60-fold volume of 60%aqueous ethanol solution was utilized to extract Punica granatum L.peel for 40 min with the assistance of sonication at 150 mA electric current intensity,and the crude extract exhibited a maximum sodium nitrite scavenging rate of 81.2%and a maximum block rate of 67.0%against the synthesis of nitrosamines,suggesting a strong ability to inhibit nitrosation.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第2期111-114,共4页
Food Science
关键词
石榴皮
总黄酮
正交试验
清除
阻断
Punica granatum L.peel
total flavonoids
orthogonal array design
scavenging
blocking