半滑舌鳎头部无眼侧皮肤表面特殊感受器的电生理反应

The electrophysiological research on the peculiar sense organs of the tongue sole(Cynoglossus semilaevis)

采用电生理学方法对半滑舌鳎头部无眼侧皮肤表面特殊感受器的感觉生理功能进行研究。结果表明,机械刺激时,无眼侧特殊感受器神经产生诱导放电信号,表明无眼侧特殊结构能对机械刺激产生反应;化学物质刺激时,随着反应浓度的增加,无眼侧特殊感受器神经的诱导信号的反应幅度和频率明显增加,表明无眼侧特殊结构对化学刺激产生反应;不同化学物质刺激强弱顺序:沙蚕提取液>L-精氨酸>L-甲硫氨酸>L-赖氨酸>L-组氨酸>二甲亚砜>L-甘氨酸>L-丙氨酸。实验表明,无眼侧特殊结构具有对机械刺激和化学刺激产生反应的双重感觉功能。据推测,这种皮肤感觉器官可能是一种与摄食有关的,在摄食中发挥积极作用的体表感觉器官。

To research the sensory physiology functions of peculiar sense organs on the abocular side of Cynoglossus semilaevis,both mechanical and chemical stimulations of the peculiar sense organs were investigated by using electrophysiological techniques.The mechanical stimulations can be demonstrated by evoking discharges from the nerves of peculiar sense organs after being touched with glass hooks lightly,which were recorded by Biomedical Multichannels Data Acquisition System.The results of small and big mechanical stimulations showed that stimulatory effects were significantly affected by the strength of mechanical stimulations;While using six kinds of L-amino acids,dimethyl sulphoxide and clam worm as chemical subjects,chemical stimulations of the dermal surface structures were also checked by the evoking discharges recorded by Biomedical Multichannels Data Acquisition System,and the frequencies and scopes of evoking discharges from the nerves of peculiar sense organs were raised as the concentrations of chemical subjects increasing.In comparison with the relations about natural responses and concentrations of chemical subjects,we knew that the order of the strength of chemical subjects was： clam worm L-Arg L-Met L-Lys L-His dimethyl sulphoxide L-Gly L-Ala.The results indicated that peculiar sense organs on the abocular side have dual sensory function to both mechanical and chemical sensitivity.Since C.semilaevis depend on sensory organs to find food,it is probable that the dermal surface structures may be relative to feeding activities,and have an active effect on feeding of tongue sole.This study develops a research about peculiar sense organs on the abocular side of C.semilaevis using electrophysiological techniques,which may serve as a valuable resource for further ultrastructure and behavioral science research of C.semilaevis.