摘要
目的 研究经颅磁刺激(TMS)对脑梗死后大鼠学习记忆功能和海马血管新生及锥体细胞树突结构的影响.方法 将48只雄性SD大鼠分为假手术组、自然恢复组和TMS组,每组16只.采用线栓法对自然恢复组和TMS组大鼠制作一侧大脑中动脉闭塞的脑梗死模型,并在制模后第2天,对TMS组给予每天2次、每次30个脉冲的TMS治疗,疗程4周;观察各组大鼠治疗后在Y-迷宫中的学习记忆成绩和梗死海马血管新生及锥体细胞树突结构变化.结果 ①TMS组大鼠学习尝试次数少于自然恢复组(P<0.01),记忆再现次数多于自然恢复组(P<0.01);②TMS组海马区微血管计数明显多于自然恢复组(P<0.05);③TMS组海马CA3区锥体细胞树突顶树突总长度长于自然恢复组(P<0.01).结论 TMS能促进脑梗死大鼠学习记忆功能的恢复,其机制可能与促进海马血管新生及锥体细胞树突结构的改变有关.
Objective To study the effects of transcranial magnetic stimulation (TMS) on learning and memory, and angiogenesis and the dendritic structure of hippocampal CA3 pyramidal neurons after cerebral infarction. Methods Forty-eight male Sprague-Dawley rats were divided into a sham operated group, a model group and a TMS group (n = 16). Rat models of focal cerebral infarction were established with unilateral middle cerebral artery (MCA) suture occlusion in the model and TMS groups. The rats of the TMS group were given 4 weeks of TMS treatment beginning 1 day after the infarction (2 times per day, 30 pulses per time). Their learning and memory abilities were tested with a Y-maze. Angiogenesis and the dendritic structure of their hippocampal CA3 pyramidal neurons were detected after 4 weeks. Results Compared with the model group, learning and memory improved significantly in the TMS group. The average microvessel density of the hippocampus in the TMS group was significantly more than in the model group. The total length of apical dendrites of hippocampal CA3 pyramidal neurons in TMS group was significantly longer than in the model group. Conclusions The improved learning and memory observed following TMS treatment are likely to be related to changes in angiogenesis, the dendritic.structure of the hippocampal CA3 pyramidal neurons, and enhanced synaptic plasticity.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2011年第1期6-9,共4页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
脑梗死
大脑中动脉
记忆
海马
毛细血管新生
经颅磁刺激
Cerebral infarction
Middle cerebral artery
Memory
Hippocampus
Angiogenesis
Transcranial magnetic stimulation