冷冻干燥^(60)钴灭菌表面脱钙异体骨移植的研究

Treatment of 31 cases of bone defects with freeze dried surface demineralized allogeneic banked bones

目的研制冷冻干燥６０钴灭菌表面脱钙异体骨并观察其治疗临床骨缺损的效果。方法切取人同种异体骨，除去骨膜和骨髓组织，用三氯甲烷甲醇和０．６ｍｏｌ／ＬＨＣｌ溶液分别脱脂、脱钙，冷冻干燥表面脱钙异体骨，并用２．５Ｍｒａｄｓ剂量的６０钴灭菌，成品骨室温下储藏。用该类型骨库骨治疗骨缺损病例３１例。其中骨病及肿瘤２０例；创伤１１例。随诊１～２０个月，平均９个月。结果所有伤口Ⅰ期愈合，术后２周最高体温的平均值为３７．８℃，骨临床愈合时间６～１６周，平均１０周，无１例发生骨不连接。结论冰冻干燥表面脱钙异体骨库骨既有一定的力学支撑能力，又有成骨诱导能力，适用于局部骨缺损的填充和关节、脊椎融合时植骨，其皮质骨能发挥部分力学支撑功能。且能室温保藏，邮政投寄，便于推广应用。

Objective\ To study and manufacture the irradation sterilized and freeze dried surface demineralized allogeneic banked bones and observe the clinical effects of treating bone defects.Method\ Thirty one patients with bone defects. of 20 with tumors and bone diseased and the remaining with traumas, were treated with irradiation sterilized and freeze dried surface demineralized allogeneic banked bones. A mean of 9 months follow up was made. The maximum of implanted bone was 100 g and minimal was 3 g .Results\ All the wounds healed well, and the mean of the patients' highest temperature 2 weeks after operation was 37.8℃. The peroids of clinical bone healing were from 6 to 16 weeks postoperatively and none of nonunion occurred.Conclusion\ Freeze dried surface demineralized bone has both osteoinductive capacity and some mechanical resistance. It is suitable to fill local bone defects and can be used in spinal and joint fusion, and can be preserved at room temperature.