苏木乙酸乙酯提取物对溃疡性结肠炎大鼠T细胞亚群和IL-6的影响

Effects of Ethyl Acetate Extract from Sappan Wood on T Cells Subsets of Rats with Ulcerative Colitis

试验旨在观察苏木乙酸乙酯提取物对溃疡性结肠炎(ulcerative colitis,UC)大鼠T细胞亚群和IL-6的影响。将40只Wistar大鼠随机分成正常组、模型组、苏木组、柳氮磺胺吡啶(SASP)组,除正常对照组外,其余各组大鼠用兔黏膜免疫法造模。造模后各组分别以生理盐水、苏木乙酸乙酯提取物、SASP灌胃给药21 d。运用流式细胞术及ELISA法检测外周血T细胞亚群和IL-6的表达。结果显示,模型组CD4+T细胞升高、CD4+/CD8+升高、IL-6的含量明显增高,与正常组相比,差异显著(P<0.05);苏木组、SASP组CD4+T细胞降低、CD4+/CD8+降低、IL-6的含量明显降低,与模型组相比,差异显著(P<0.05);苏木组与SASP组相比,差异不显著(P>0.05)。试验结果证明,苏木乙酸乙酯提取物通过调整T细胞亚群和降低IL-6含量,发挥对UC大鼠的治疗作用。

To observe the effect of ethyl acetate extract from sappan wood on T lymphocyte subsets and interleukin-6 of rats with ulcerative colitis（UC）,40 Wister rats were randomly divided into normal group,model group,sappan wood group and Sulfasalazine（SASP） group.Ulcerative colitis was induced by immunization with rabbit＇s colonic mucous emulsified with completely Freund＇s adjuvant in rats except normal group.The rats were respectively treated with enemas as follows physiological saline,ethyl acetate extract from sappan wood and SASP for 21 days.We analyzed expression of T lymphocyte subsets from peripheral blood with flow cytometry（FCAS methods） and measured the IL-6 contents in serum by enzyme linked immunosorbent assay（ELISA methods）.Results displayed that compared with normal group,CD4＋ T lymphocytes,CD4＋/CD8＋ ratio and the IL-6 contents were increased significantly in model group,compared with model group,CD4＋ T lymphocytes,CD4＋/CD8＋ ratio and IL-6 content were reduced significantly in sappan wood group and SASP group,and no significant difference could be demonstrated between sappan wood group and SASP group.Evidences demonstrated that the ethyl acetate extract from sappan wood may regulate T lymphocyte subsets and reduce the contents of IL-6,consequently play a role of protection in rats with ulcerative colitis.