流动注射在线固相萃取预富集毛细管区带电泳测定血浆中的伪麻黄碱

Determination of Pseudoephedrine in Human Plasma Using Capillary Zone Electrophoresis Coupled by Flow Injection on line Solid Phase Extraction

研究了流动注射在线固相萃取与毛细管区带电泳联用系统（ＦＩ－ＳＰＥ－ＣＺＥ）应用于测定血浆中痕量盐酸伪麻黄碱的方法． 通过脱线和在线试样净化、优化ＣＺＥ分离条件等措施消除了血浆中共存组份的干扰； 通过增加固相萃取的进样量、加大场放大电动进样量和柱上堆积的力度等，提高了联用系统的灵敏度． 血浆试样沉淀蛋白后可连续进入ＦＩ－ＳＰＥ－ＣＺＥ系统进行自动的浓缩、分离与分析， 检测限为１２ ｎｇ／ｍ Ｌ， 分析速度达１０ 次／ｈ． 应用本方法成功地测定了志愿者口服正常剂量盐酸伪麻黄碱后的血药浓度，

The previously developed hyphenated system of flow injection on line solid phase extraction capillary zone electrophoresis(FI SPE CZE) was adapted to the determination of trace pseudoephedrine in human plasma. Interference from the coexisting species was eliminated by off line and on line sample cleaning up, and by optimization of CZE separation conditions. The sensitivity of hyphenated system was improved by increasing of loaded sample volume and strengthening of field amplified stacking effect. Thus, plasma samples, after deproteinized by off line precipitation, could be consecutively loaded onto the FI SPE CZE system, and trace amounts of pseudoephedrine be automatically preconcentrated, separated and determined by the system. The adapted FI SPE CZE system was successfully applied to determination of trace pseudoephedrine in volunteer s plasma samples for pharmacokinetic studies after administration of the drug with normal dosage.