摘要
目的:测定抗孕唑对假孕大鼠黄体和蜕膜的作用,以探索抗孕唑抗早孕作用的机制。方法:用放射免疫法和紫外分光光度计等方法。结果:抗孕唑可缩短大鼠假孕期48d;降低卵巢重量,蛋白含量和血清孕酮浓度;给药5d后,3βHSD活性显著低于对照组;20αHSD的活性则在给药1d后即非常显著上升;子宫PGF2α含量也显著升高,而给药5d后,子宫和血清中的PGF2α分别为对照组的32倍和22倍。结论:抗孕唑的溶黄体作用是由于降低假孕大鼠血清孕酮水平和增加PGF2α含量所致,似乎与胚胎坏死无关。
AIM: To observe the luteolytic effects of DL111IT in pseudopregnant rats. METHODS: Radioimmunoassay was used to estimate serum progesterone concentration and PGF2 content in uterus and serum; the activities of 3HSD and 20HSD in ovaries were assayed spectrophotometrically. RESULTS: DL111IT was shown to shorten normal pseudopregnant period of rats by 48 days and decrease ovarian fresh weight, protein content and serum progesterone level dramatically. After im DL111IT for 5 d, activities of 3HSD of pseudopregnant rats decreased 538%, while that of 20HSD incresed significantly after only one injection of DL111IT (control: 1004 mumg-1 protein; treatment: 6918 mumg-1 protein); PGF2 concentration in uterus and serum of pseudopregnant rats increased 401% and 365%, respectively. CONCLUSION: DL111IT induced luteolysis in pseudopregnant rats.
出处
《药学学报》
CAS
CSCD
北大核心
1999年第5期321-324,共4页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
中国科学院上海药物研究所新药研究国家重点实验室资助