高效液相色谱法测定布洛芬与人血浆蛋白的结合率

High performance liquid chromatographic determination of ibuprofen concentration and the binding rate of ibuprofen to human plasma proteins

目的:建立人血浆中布洛芬浓度的测定方法,测定布洛芬在人血浆中的蛋白结合率,并计算相关参数。方法:用平衡透析法以高效液相色谱法为检测手段测定血浆蛋白结合率。结果:布洛芬与人标准血浆的蛋白结合率为(95.4±0.3)%。结论:布洛芬与人血浆蛋白为高强度结合。

OBJECTIVE To develop a high performance liquid chromatographic（HPLC） method to determine the concentration of ibuprofen in human plasma,and study the binding rate of ibuprofen to plasma proteins and to calculate the binding parameters of ibuprofen to plasma proteins. METHODS The binding rate of ibuprofen to human plasma proteins was determined by equilibrium dialysis method and HPLC method. RESULTS The binding rate of ibuprofen to human plasma proteins was （95.4±0. 3）%. CONCLUSION The binding rate of ibuprofen to human plasma proteins is high strength.