棉籽蛋白发酵制备ACE抑制肽条件的优化

Optimization of Fermentation Conditions in Production of ACE Inhibitory Peptides From Cotton Seed Protein

以棉籽蛋白为原料,采用枯草芽孢杆菌NCT314发酵制备血管紧张素转化酶(ACE)抑制肽。首先从温度、时间、pH和接种量4个方面研究了制备棉籽蛋白ACE抑制肽的单因素条件;然后在单因素试验的基础上,运用Box-Behnken的中心组合试验设计原理,采用响应面分析,对发酵条件进行了优化。优化后的发酵条件为温度32.08℃、时间22.6 h、pH为7.2,在该条件下,发酵液的ACE抑制率最高可达78.06%,IC50值达6.67 mg/mL.

Angiotensin-I-converting enzyme（ACE） inhibitory peptide was derived from cotton seed protein fermented with Bacillus subtilis NCT314.The fermentation conditions including temperature,time,pH and inoculum size were investigated.Based on the single factor experiments,the fermentation condition was optimized by response surface methodology.The result showed the optimum fermentation condition was temperature 32.08℃,time 22.6 h and pH 7.2.Under this condition,the ACE inhibitory ratio of fermented liquid reached to 78.06 %,and IC50 value was 6.67 mg/ml.