香蕉皮多糖对人乳腺癌细胞增殖、克隆形成与核因子-κB蛋白表达的影响

Influence of Polysaccharides of Banana Peel on Proliferation,Colony Formation and Expression of Nuclear Factor-κB in Human Breast Cancer Cells

目的:观察香蕉皮多糖对体外培养的人乳腺癌细胞增殖、克隆形成及其核因子-κB(nudear-kappa B,NF-κB)p65蛋白表达的影响。方法:采用溴化四甲基偶氮[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,MTT]法,取对数生长期人乳腺癌细胞(MCF-7),随机分为药物组与对照组,用质量浓度为0.5,1.5,2.5,3.5 g·L-1香蕉皮多糖作用于MCF-7细胞,分别干预24,48,72 h。通过克隆形成试验,Western blot试验,观察香蕉皮多糖对MCF-7细胞克隆形成及对MCF-7细胞NF-κB(p65)蛋白表达的影响,试验中设置药物组与对照组,药物组MCF-7细胞加入质量浓度为0.5,1.5,2.5 g·L-1香蕉皮多糖干预48 h。结果:香蕉皮多糖对MCF-7细胞的增殖有抑制作用,诱导肿瘤细胞凋亡作用与药物浓度和药物作用时间成正相关。MCF-7细胞的克隆形成率,NF-κB(p65)蛋白的表达随香蕉皮多糖浓度升高而显著下降。结论:香蕉皮多糖对MCF-7细胞增殖,克隆形成有抑制作用,(P<0.05)。香蕉皮多糖干预后,MCF-7细胞表达NF-κB(p65)明显减弱。其诱导MCF-7细胞凋亡作用机制可能下调NF-κB表达水平有关。

Objective：To study the effect of Polysaccharides of Banana Peel on proliferation,colony formation and expression of nuclear factor-kappa B（NF-κB）p65 in human breast cancer cells.Method：The MTT assay was used,and the human breast cancer cells（MCF-7） in logarithmic phase of growth were randomly divided into drug group and control group,with the concentration of 0.5,1.5,2.5,3.5 g·L^-1 Banana Peel polysaccharides in MCF-7 cells,Intervention durations were 24,48,72 h accordingly.The colony formation assay and the western blot technique were used to observe the effects of Polysaccharides on human breast cancer cell colony formation and expression of NF-κB（p65） in breast cancer cells.Test set of drug group and control group,drug group MCF-7 cell with the concentration of 0.5,1.5,2.5 g·L^-1 Banana Peel polysaccharides intervention 48 h.Result：Banana Peel polysaccharides inhibited cell proliferation and induced apoptosis of tumor cells within the drug concentration and drug effects duration of studies.MCF-7 cell colony formation rate,NF-κB（p65） protein expression decreased significantly with a dose-response manner Conclusion：Banana peel polysaccharides inhibited the MCF-7 cellsproliferation and colony formation（P 〈 0.05）.Polysaccharide banana peel down-regulated the expression levels of NF-κB（p65） protein in MCF-7 cells.The mechanism of MCF-7 apoptosis may be related to down-regulation of the expression levels of NF-κB（p65）.