泥沙颗粒生长生物膜前后表面变化的试验研究

Experiment of particles’morphology variation after biofilm growth on sediments

以泥沙颗粒为载体,以天然水配置营养液进行生物膜培养试验,采用环境扫描电子显微镜观察泥沙颗粒生长生物膜前后的形貌变化,并用图像处理和梯度计算的方法对获得的灰度图片进行分析,发现泥沙颗粒因生物膜的生成而发生较大变化,群体泥沙互相粘连,形成网状结构或絮状结构等,单颗粒泥沙因为生物膜的存在使得长膜沙相比原状沙其表面导数更多地集中于0值附近,其中,X方向梯度幅度特征值相差4.11%,Y方向梯度幅度特征值相差2.19%,二阶导数梯度幅度特征值相差28.84%,说明泥沙颗粒长膜之后表面比较平滑,形貌起伏相对较少。

In this paper, sediment particles are used as carriers, natural water is configured to nutrient water to cultivate biofilm. The environmental scanning electron microscope is used to capture the particle surface morphology variation after biofilm growth on sediment. Analysis on obtained gray images shows that the sediment surface morphology has changed greatly because of the biofilm growth. Sediment group becomes co-adhesive and forms reticular structure or floc structure. The surface derivative of biofilm sediment is more concentrated to zero than that of original biofilm, in which, the difference of gradient amplitude characteristics in X direction and Y direction are 4.11% and 2.19% respectively,and the difference of second-derivative amplitude characteristics is 28.84%. All these show that the surface micro morphology of sediment particles after biofilm growth becomes smoother.