HPLC法测定复方红景天胶囊中红景天苷的含量

Determination of salidroside in compound hongjingtian capsules by HPLC

目的建立复方红景天胶囊中红景天苷的含量测定方法。方法样品采用甲醇超声提取的方法制备。色谱条件采用Shim-Pack ODS柱(150 mm×4.6 mm5,μm),甲醇-水(10∶90)为流动相,检测波长为223 nm。结果红景天苷浓度在9.61～96.08 mg.L-1范围内与峰面积值呈良好的线性关系(r=0.999 9)。回收率为97.34%,RSD=1.02%(n=6)。结论该法准确、可靠、稳定,可作为复方红景天胶囊的定量分析方法。

Aim To develop a method for determination of salidroside in compound hongjingtian capsules.Methods The sample was prepared in methonal by ultrasonic extraction.The analysis was performed on a Shim-Pack ODS column,with a mobile phase of methanol-water（10∶90）,the detection wavelength set at 223 nm.Results The linearity of the concentration of salidroside and its peak area was fine in the range of 9.61 ～96.08 mg·L-1（r=0.999 9）.Recovery of salidroside was 97.34%,and RSD was 1.02%.Conclusion The method is accurate,reliable,stable and it can be used for the quantitative analysis of salidroside in compound hongjingtian capsules.