健脾化瘀方中各味中药对肠癌细胞株CEA表达的影响

Effect of Every Chinese Herb in Jianpi Huayu Recipe on the CEA of the Colon Cancer cell

目的:探讨健脾化瘀方中各中药对肠癌细胞株癌胚抗原(CEA)表达的影响。方法:用亚细胞毒浓度的药物作用于肠癌细胞一定时间后分别收集细胞培养液上清和细胞,分别采用夹心ELISA法和免疫组化法检测肠癌细胞培养上清CEA的表达。结果:健脾化瘀方中各药均具有不同程度的抑制肠癌细胞生长作用,并且随着中药浓度的递减,抑制率也相应的减低;夹心ELISA法检测培养上清液CEA的表达在24h时,蚤休及制军对CEA的浓度有明显的降低作用,有统计学意义(P<0.05);免疫组化检测CEA的表达显示:与对照组相比,蚤休、制军对CEA表达的阳性染色率最低,虎杖、旋复花阳性染色率相当,丹参、白术阳性染色率最强。结论:健脾化瘀方中各药均有抑制肠癌细胞株CEA表达的作用。

Objective：To study the effect of every Chinese herb in Jianpi Huayu Recipe on the CEA of the colon cancer cell.Methods： Sandwich ELISA and Immunohistochemical detection were used to detect the CEA of the cancer cell culture supernatant.Results： Chinese herbs of Jianpi Huayu Recipe all could inhabit the growth of colon cancer cell,and with the decreasing concentration of herbs,effect correspondingly also reduced.Used the method of Sandwich ELISA and immunohistochemical detection,the expression of CEA can be decreased by all herbs.ZhiJun and ZaoXiu were the most significant in reduc the expression of CEA.Conclusion： All Chinese herbs of Jianpi Huayu Recipe can inhibit the expression of CEA of the colon cancer cell.