骨质疏松大鼠膝关节组织总胶原、Ⅰ型及Ⅱ型胶原蛋白的含量变化

Fluctuation of total collagen,type Ⅰ and Ⅱ collagen protein contents in the knee joint of osteoporotic rats

背景:目前研究认为胶原蛋白在骨细胞的增殖与分化、骨质的形成和吸收、骨基质矿化等过程中起着非常重要的作用。目的:观察骨质疏松模型大鼠膝关节组织总胶原、Ⅰ型及Ⅱ型胶原蛋白含量变化。方法:SD雌性大鼠40只随机分为实验组和对照组,实验组大鼠行双侧卵巢切除16周后,取膝关节软骨和交叉韧带,采用微量羟脯氨酸测定法及酶联免疫吸附法测定大鼠膝关节软骨及前交叉韧带组织总胶原、Ⅰ型及Ⅱ型胶原蛋白含量。结果与结论:骨质疏松膝关节模型大鼠膝关节软骨、前交叉韧带总胶原、Ⅰ型及Ⅱ型胶原蛋白含量明显下降(P<0.05),Ⅰ型/Ⅱ型胶原比值也显著降低(P<0.05),提示骨质疏松可引起膝关节内组织总胶原、Ⅰ型及Ⅱ型胶原含量及其比值改变,与膝关节骨性关节炎的发生密切相关。

BACKGROUND：Studies demonstrated that collagen protein plays an important role in osteocytes proliferation and differentiation,sclerotic formation and absorption,as well as matrixmineralization.OBJECTIVE：To observe the fluctuation of total collagen,type Ⅰ and Ⅱ collagen protein contents in knee joint of osteoporotic rats.METHODS：Totally 40 female Sprague Dawley rats were randomly assigned into the experimental and control groups.In the experimental group,rat knee joint cartilage and cruciate ligament were harvested at 16 weeks after bilateral ovariectomy.The protein contents of total,type Ⅰ and Ⅱ collagen were measured by micro-hydroxyproline assay and enzyme linked immunosorbent assay.RESULTS AND CONCLUSION：Compared with the control group,the protein contents of total,type Ⅰ and Ⅱ collagen were notably decreased （P0.05）,and the type Ⅰ/Ⅱ collagen ratio was also declined in the experimental group （P0.05）.The results suggested that osteoporosis leads to fluctuations of total collagen,type Ⅰ,Ⅱ collagen protein contents and type I/II collagen ratio in knee joints,which closely relates to knee osteoarthritis.