近红外荧光量子点对颊癌可视化活体

Visual imaging for buccal carcinoma with near-infrared fluorescent quantum dots in vivo

目的 观察用最大发射波长为800 nm近红外荧光量子点（quantum dots,QD800）标记的昆明小鼠鳞状细胞癌U14细胞在体内颊部的可视化成像情况,为癌症的早期诊断、可视化观察和个体化治疗提供依据.方法 用具有穿膜作用肽段连接的QD800通过内吞标记U14细胞（U14-QD800）,将含有不同细胞数的U14-QD800分别注射于裸鼠和昆明小鼠颊部黏膜下,在不同时间行活体成像,观察用QD800标记对U14细胞非侵入体内检测的敏感性和动态成像情况.结果 活体成像对裸鼠和昆明小鼠颊部可视化检测到的最少U 14-QD800细胞数分别为1×104和1×105个.1×104、1×105和1×106个U14-QD800细胞在裸鼠颊部能可视化成像持续的时间分别为3、7和16 d,1×105和1×106个U14-QD800细胞在昆明小鼠颊部能可视化成像持续的时间分别为3和10 d.结论 由于近红外荧光量子点对组织具有强的穿透力,在癌症的早期诊断、可视化观察和个体化治疗方面具有较大的发展前景.

Objective To examine the in vivo visual imaging of buccal carcinoma with the nearinfrared fluorescent quantum dots. Methods The U14 cells were labeled by endocytosis with QD800 （U14/QD800） which was linked with cell-penetrating peptide. Different number of U14/QD800 was injected under the buccal mucosa of nude mice and Kunming mice separately and imaged at different time to detect the in vivo sensitivity and dynamic imaging of U14/QD800. Results The minimum number of U14/QD800 cells which could be detected by in vivo imaging system was 1 × 104 in nude mice＇s cheek and 1 × 105 in Kunming mice＇s. The time for visual imaging of 1 × 104, 1 × 105 and 1 × 106 U14/QD800 cells in nude mice was 3, 7 and 16 d separately, and 3 and 10 d separately in Kunming mice. Conclusions Due to its strong tissue penetration, near-infrared fluorescent quantum dots have great prospects in cancer early diagnosis, visual observation and individual treatment.