产辅酶Q_(10)白地霉菌的罐发酵条件优化

Optimization of Tank Fermentation Conditions for Coenzyme Q_(10) Production by White Geotrichum candidum

以白地霉菌(Geotrichum candidum)为出发菌株,根据摇瓶发酵结果控制pH值进行罐发酵。采用间歇补料分批发酵的方法,考察溶氧量、搅拌转速、空气流量及补糖因素对菌体生物量及辅酶Q10产量的影响。确定发酵罐发酵的最适条件为控制溶氧在35%～40%,补糖控制在发酵液糖质量浓度为2g/L,该发酵条件下辅酶Q10产量从分批发酵的78.75mg/L提高到106.26mg/L,较摇瓶发酵提高了69.8%。

For the production of Coenzyme Q10,Geotrichum candidum was intermittent fed-batch cultured in a stirred tank fermenter,and pH was controlled according to the results of previous studies on shake flask fermentation during the process.The effects of dissolved oxygen,stirring speed,airflow rate,and sugar amount on biomass and coenzyme Q10 production were investigated.The optimal fermentation conditions were determined as follows：dissolved oxygen,35%-40% and sugar concentration,2 g/L.Under these optimal conditions,the yield of coenzyme Q10 from batch fermentation was increased from 78.75 to 106.26 mg/L,which exhibited an improvement by 69.8% as compared to shake flask fermentation.