陕西地区造血干细胞捐献者人类白细胞抗原HLA等位基因频率分析

Allele frequency of human leukocyte antigen among hematopoietic stem cell donors in Shanxi province

目的:了解陕西地区人类白细胞抗原(HLA)-A、B、DRB1等位基因分布的特征。方法:采用PCR-SSP和PCR-SSOP方法对10122名造血干细胞捐献者样本进行HLA-A、B、DRB1等位基因分型分析。结果:鉴定了20个A等位基因,34个B等位基因,14个DRB1等位基因。频率最高的基因型分别为A*02、B*15、DRB1*15,其相应基因频率分别为30.03%、14.09%和15.51%。结论:陕西地区人群HLA-A、B、DRB1等位基因频率具有较丰富的多态性。

Objective：To investigate the characteristics of allele distribution of HLA-A、B、DRB1 in Shanxi province.Method：10122 blood samples of hematopoietic stem cell donors who registered in Shanxi branch of Chinese Marrow Donor Program were analyzed for the allele of HLA-A,B,DRB1 using PCR-SSP and PCR-SSOP techniques.Result：A＊02（30.03%）,B＊15（14.09%） and DRB1＊15（15.51%） had the highest frequent distribution among the allele of HLA-A,B,DRB1 in Shanxi donors.Conclusion：There would be abundant allele polymorphisms in Shanxi population.