三七配方颗粒的HPLC指纹图谱研究

Study on HPLC Fingerprint Analysis of Notoginseng Radix et Rhizoma Formula Granules

目的:建立三七配方颗粒的HPLC指纹图谱,为科学评价其质量提供依据。方法:采用高效液相色谱法,以Waters Xbridge C18柱(4.6 mm×250 mm,5μm)为色谱柱,以乙腈-水为流动相梯度洗脱,检测波长203 nm,流速1.0 mL.min-1,柱温30℃。结果:建立了三七配方颗粒的指纹图谱,确定了18个共有峰,各三七配方颗粒样品指纹图谱与对照指纹图谱相似度均在0.9以上,可用于三七配方颗粒的定性鉴别。结论:该方法简单、准确、重复性好,能有效地对三七配方颗粒的质量进行评价。

Objective：To establish a method of fingerprint analysis on Notoginseng Radix et Rhizoma Formula granules by HPLC and provide a reference for quality evaluation of Notoginseng Radix et Rhizoma Formula granules.Method： The chromatographic fingerprints were obtained by the Waters Xbridge C18 column（4.6 mm × 250 mm,5 μm） with the gradient elution and the solvent system was composed of acetonitrile-water.The detective wavelength was set at 203 nm;the flow rate was 1.0 mL.min-1 and the column temperature was maintained at 30 ℃.Result：Eighteen copossessing peaks were selected as the fingerprint peaks of Notoginseng Radix et Rhizoma Formula granules and good similarities with correlation coefficients higher than 0.9 were found in fingerprints between the different samples of Notoginseng Radix et Rhizoma Formula granules and the standard fingerprint.Conclusion： The method with good reproducibility is simple and accurate;it can be used as a quality control method for Notoginseng Radix et Rhizoma Formula granules.