肝炎病人血清外周血单核细胞及肝脏中GBV-C/HGV负链RNA的检测

Detection of minus-stranded GBV-C/HGV RNA in the serum, liver and peripheral blood mononuclear cells in patients with hepatitis

目的：研究27例肝炎病人血清、外周血单核细胞（PBMC）及肝脏中GBV－C／HGV正链及负链RNA（复制中间体）的存在状况。方法：应用逆转录-巢式多聚酶链反应技术检测GBV－C／HGV正、负链RNA。结果：27例病人中21例病人血清、7例PBMC、10例肝组织中检测到GBV－C／HGV正链RNA，其中2例单一GBV－C／HGV感染者肝组织中检测到负链RNA，在27例病人血清及PBMC中均未检测到负链RNA。结论：GBV－C／HGV是一种嗜肝病毒，肝脏可能是其复制的主要场所之一，但GBV－C／HGV与HCV混合感染对，在PBMC及肝脏中尚未发现该病毒复制的证据。

Objective: To investigate the presence of plus-and minus-stranded HGV RNA in the serum,liver and peripheral blood mononuclear cells (PBMC). Methods: Plus-and minus-stranded HGV RNA wasdetected with reverse-transcription nested polymerase chain reaction in 27 patients with hepatitis. Results:Plus-stranded HGV RNA was found in 21 cases in serum 10 cases in liver sample and 7 cases in PBMC. Nominus stranded HGV RNA was found in serum and PBMC. Minus-stranded HGV RNA was detectal in 2cases infected only with GBV-C/HGV in the liver sample. Plus-stranded HGV RNA was found in 10 of 21liver samples from the patients with positive detection of HGV RNA in the serum and 2 of minus-strandedpositive samples. No minus-stranded HGV RNA was found in the serum and PBMC. ConclusiOn: GBV-C/HGV is a hepatotropic virus that replicates in the human liver.