大豆子叶节植株再生体系的研究

Research on Cotyledonary Nodes Regeneration System of Soybean

以大豆品种"辽豆17"、"辽豆18"和"辽豆23"子叶节为外植体诱导不定芽的发生。结果表明:随着培养基中6-BA浓度的增加,每块外植体上不定芽的数量呈增加的趋势,当向1/2MSB培养基中添加0.05 mg·L-1IBA和4.0 mg·L-16-BA时,不定芽的诱导率最高。比较不同的外植体获得方法,保留半片子叶的外植体诱导丛生芽的效果好于不保留子叶的外植体。1/2 MSB+0.5 mg·L-16-BA+0.05 mg·L-1IBA是适宜的不定芽伸长培养基。1/2MSB+1.0 mg·L-1IBA是适宜的生根培养基。

The adventive shoots were induced from cotyledon nodes with soybean cultivar Liaodou 17,Liaodou 18 and Liaodou 23 as explants.With the increase of 6-BA concentration,the number of adventitious shoots on each explant showed an increasing trend,when 0.05 mg·L^-1 IBA and 4.0 mg·L^-1 6-BA were added into 1/2MSB medium,the induction rate of adventive shoots is the highest.Compared the effect of explants,the explants of reserving half cotyledon was better than the cotyledon nodes without cotyledon.Therefore,the optimum culture medium for elongation of adventitious bud was 1/2MSB＋0.5 mg·L^-1 6-BA＋0.05 mg·L^-1 IBA,and the optimum culture medium for rooting was 1/2MSB＋1.0 mg·L^-1 IBA.