犬体外腹主动脉末端阻断再灌注损伤指标的变化及意义

The variation and significance of plasma superoxide dismutase and malondialdehyde in canine with ischemia-reperfusion injury by abdominal aorta block in vitro

目的:观察正常犬行体外腹主动脉末端阻断缺血再灌注损伤指标变化,为在急救时临时阻断腹主动脉控制出血提供实验依据。方法:犬麻醉后气管插管。自制体外腹主动脉阻断装置将腹主动脉远段向脊椎椎体挤压至血流完全阻断,阻断时间30min。分别于腹主动脉阻断前、阻断后30min及开放阻断后即刻、开放阻断后30、120、240min各时间点由前肢抽取静脉血。测定血浆超氧化物歧化酶(SOD)、血浆丙二醛(MDA)含量。结果:动脉开放即刻、30、120min血清SOD活力下降,与阻断前基础值比较差异有显著性(P<0.05)。动脉开放30、120min血清MDA值升高,与阻断前基础值相比差异有显著性(P<0.05)。结论:犬体外腹主动脉末端阻断30min,动脉开放后引起的缺血再灌注损伤局限在短时间可恢复的程度。

Objective To observe the variation and significance of plasma superoxide dismutase （SOD） and plasma malondialdehyde （MDA） in canine with ischemia-reperfusion injury caused by abdominal aorta block in vitro, and provide experimental basis for temporary control of hemorrhage in emergency situation. Methods Abdominal aorta block was carried out in five dogs for 30 min with homemade device after anesthesia, the contents of SOD and MDA were determined before and after abdominal aorta block, at instantly, 30, 120 and 240 rain after reperfusion. Results Compared with base value, the contents of SOD were lower at instantly, 30, 120 min after reperfusion （P 〈 0.05）, and the contents of MDA were higher at 30, 120 rain after reperfusion. Conclusions The ischemia-reperfusion injury caused by 30-min abdominal aorta block can recover in a short time.