摘要
目的:建立豚鼠形觉剥夺性近视动物模型,观察豚鼠视网膜超微结构变化,研究环磷酸鸟苷(cyclic guanine monophosphate,cGMP)在近视豚鼠视网膜组织上的表达变化,探讨其发病机制。方法:3周龄的三色豚鼠随机分为未遮盖组(Ⅰ组)、单眼遮盖2周组(Ⅱ组)和单眼遮盖3周组(Ⅲ组),其中右眼遮盖为实验眼,左眼为自身对照眼。对各组进行视网膜检影和A超测眼轴。电子显微镜检测视网膜超微结构变化,放射免疫法检测cGMP的含量。结果:豚鼠形觉剥夺后近视形成,眼轴延长,视网膜超微结构随形觉剥夺时间延长,眼底病理性改变加重;实验眼视网膜上cGMP随形觉剥夺时间的延长明显上调,与自身对照眼比较差异有统计学意义(P<0.05)。结论:对豚鼠进行无创性眼罩遮盖可建立有效的形觉剥夺性近视模型。视网膜上cGMP含量的改变可能在近视发病机制中起重要作用。
Objective To explore the expression of cyclic guanine monophosphate(cGMP) and the ultrastructure change in retina of guinea pig with form-deprivation myopia and the underlying mechanisms.Methods Three-weeks-old guinea pigs were distributed in 3 groups: an untreated group(Group Ⅰ),a mypia 2-weeks group(Group Ⅱ) and a mypia 3-weeks group(Group Ⅲ),animals underwent monocular form-deprivation by facemask for 2 and 3 weeks.The right eyes were deprived and the left eyes were self-controlled.The refraction and axial length of the eyes was measured.Retina was observed by electron microscope.The expression of cGMP was detected by radioimmunochemistry.Results Deprived eyes in guinea pig showed significant development of myopia,the refraction and axial length was increased.The pathological changes in ultrastructure of retina were aggravated with the development of myopia.The expression of cGMP was significantly up-regulated in the deprived eyes compared with self-control eyes(P0.05).Conclusion Form-deprivation can up-regulate the expression of cyclic GMP,which might play an important role in the development of myopia.
出处
《中南大学学报(医学版)》
CAS
CSCD
北大核心
2011年第3期249-253,共5页
Journal of Central South University :Medical Science
基金
湖南省自然科学基金(09JJ5033)
湖南省科技厅科技计划项目(2009FJ3175
2010SK3125)~~
关键词
环磷酸鸟苷
形觉剥夺性近视
视网膜
豚鼠
cyclic guanine monophosphate
form-deprivation myopia
retina
guinea pig