慢性阻塞性肺疾病患者诱导痰和血清中白细胞介素-13水平相关性研究

Relationship between Interleukin-13 in Plasma and Induced Sputum in Chronic Obstructive Pulmonary Disease

目的观察慢性阻塞性肺疾病(COPD)患者诱导痰及血清中白细胞介素-13(IL-13)水平的变化。方法 COPD急性加重期患者30例(加重组)、COPD稳定期患者30例(稳定组)、健康体检者25例(对照组),采用酶联免疫吸附法(ELLSA)检测诱导痰及血清中IL-13水平,并检测患者肺功能。结果加重组及稳定组诱导痰及血清中IL-13水平均高于对照组(P<0.01),加重组诱导痰及血清中IL-13水平高于稳定组(P<0.01);血清IL-13水平与肺功能无相关性(P>0.01),诱导痰IL-13水平与肺功能呈负相关性(P<0.01)。结论 COPD患者诱导痰及血清中IL-13水平增高,与COPD的发生及加重密切相关。全身炎症反应与气流受限关系不大,而局部炎症反应与气流受限密切相关。

Objective To study changes in levels of IL-13 in induced sputum and plasma in patients with chronic obstructive pulmonary disease（COPD）.Methods Levels of IL-13 in induced sputum and plasma and pulmonary functionwere examined for patients with COPD during acute exacerbation and stable stage.Healthy volunteers were included as controls.Results Levels of IL-13 in patients with COPD in induced sputum and plasma were significantly higher than those in healthy controls（P0.01）.The concentrations of IL-13 in patients with COPD during acute exacerbation were significantly higher than those during stable stage（P0.01）.The 1evels of IL-13 in plasma was not correlated with their pulmonary function（P0.01）,but the levels of IL-13 in induced sputum was negatively correlated with their pulmonary function（P0.01）.Conclusion The levels of IL-13 in induced sputum and plasma of patients with COPD increase,which may be related with the pathogenesis and exacerbation of COPD.Local inflammatory reactions,rather than systematic one,seem to be closely related to airflow limitation in patients with COPD.