三种神经功能评分在鼠类局灶性脑缺血模型评价中的比较

Comparison of three kinds of neurological function rating systems on focal cerebral ischemia models of rodent animals

目的:比较Garcia JH、Clark和Bederson3种神经功能评分对鼠类局灶性脑缺血模型的评价意义。方法:采用线栓法阻塞大鼠和小鼠大脑中动脉,造成局灶脑缺血模型,并采用脑缺血治疗药物清开灵注射液进行干预。24h后,分别采用3种神经功能评分方法进行评价,测定脑梗死体积比例;分析神经功能评分与脑梗死体积比例的相关性及不同评分间的相关性,并进行比较。结果:大脑中动脉阻塞模型(MCAO)术后,动物均出现了脑梗死病灶及神经功能的损伤,清开灵注射液治疗可显著减小脑梗死体积并改善神经功能。采用相关性分析发现,大鼠实验中GarciaJH评分和Bederson评分与梗死体积显著相关,且两种神经评分之间显著相关;小鼠实验Clark局灶性功能损伤评分与梗死体积显著相关,Bederson评分和Clark一般功能损伤评分未显示与脑梗死体积相关,但局灶性功能损伤评分与一般功能损伤评分和Bederson评分均显著相关。结论:同Bederson评分相比,Garcia JH评分和Clark局灶性功能损伤评分与脑梗死体积具有更好的相关性,更适合作为脑缺血治疗药物疗效评价的指标。

Objective： To compare 3 kinds of neurological function rating systems, Garcia JH＇s, Clark＇s and Bederson＇s, on rodent models with focal cerebral ischemia. Method： After cerebral ischemia models were made by middle cerebral artery occlusion （MCAO） with nylon thread in rats and mice. Qingkailing, an effective medicine for cerebral ischemia, was administrated to animals. In 24h, 3 kinds of rating systems were employed to evaluate neurological functions of rats and mice. Meanwhile, the infarction volumes of animal brain were calculated by TTC staining and computer-aided image analysis. The correlations between neurological function scores and brain infarction volumes were calculated and compared with SPSS 16.0. Result： After MCAO, cerebral infarction lesions and neurological functions deficit were manifested. Qingkailing was proved to be effective on improving neurological functions and decreasing infarction lesions on MCAO animals. Comparison of 3 kinds of neurological function rating systems showed that Garcia JH＇s and Bederson＇s scoring system were both correlated with infarction volume significantly in rats, and both of them were correlated with each other significantly; Clark＇s focal symptoms scoring showed significant correlation with infarction volume in mice; Be derson＇s scoring and Clark＇s whole symptoms scoring were not correlated with cerebral infarction volume, however, both focal symptoms scoring and whole symptoms scoring were correlated with Bederson＇s scoring system significantly. Conclusion： Compared with Bederson＇s scoring system, Garcia JH＇s and Clark＇s focal scoring systems showed better correlation with cerebral infarction volume, which suggested latter two scoring systems are more suitable for evaluating drug efficacy.